Microscope type:   LM ► Optical Microscopy ► CLSM    back to list

 

Olympus Fluoview-1000 confocal with Two-Photon excitation

 

 

Microscope:Olympus IX 81 inverted
Lasers:
  • CNI solid-state lasers: 405nm/30mW
  • 473nm/10mW
  • 561nm/20mW
  • Detector:3 PMTs, plus 2 GaAsP high sensitivity PMTs, transmission detector
    Lenses:
  • UPLSAPO 60x water, NA:1.2
  • UPLFLN 40x oil, NA:1.3
  • UPLSAPO 10x air, NA:0.40
  • Filters:490-540nm, 490-590nm, 585-685nm
    Additional equipment:
  • Ti-sapphire laser (Mira/Verdi 8 Watt combo) for two-photon excitation.
  • Simultaneous second scanner.
  • Xenon short-arc high-power flash-lamp for UV-flash photolysis.
  • Patch-clamp amplifier for whole-cell and single channel voltage clamping.
  • Rapid superfusion system (5 solution lines).
  • Special application:Confocal Ca2+ imaging with fluorescent indicators (fluo-3, fluo-5N, rhod-2), can be combined with UV-flash and two-photon excitation photolysis of caged compounds.
    Contact:Prof. Dr. Ernst Niggli
    Location:Department of Physiology
    University of Bern
    Bühlplatz 5
    3012 Bern
    More information



    Special Applications:
  • Multi-Photon



  • Last update:14.06.2017 / Ernst Niggli